Composite

Part:BBa_K2401000:Design

Designed by: Shanti Ricke   Group: iGEM17_Hamburg   (2017-10-19)


Synthethases + Activators for Pyochelin and Yersiniabactin siderophor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 8003
    Illegal SapI site found at 8029


Design Notes

During the design process we had to care that some genes are overlap in our construct like they do in the natural sequence. Since this biobrick consists of several protein coding sequences that only work together in a ratio 1:1:1:1, we had to make sure that they are all promoted evenly and the transcription proceeds as smoothly as possible.


Source

Genomic Sequence of K. Pneumonia

References